| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.043488 |
| Chromosome: | chromosome 11 |
| Location: | 544236 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467601 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGCTTCAATGTCATCAACGAGAACAACGTGGAGGTGGAGATCAAGGA |
| Internal bar code: | TTAACACAGGCGGTCTTGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1157 |
| LEAP-Seq percent confirming: | 23.4375 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGCAGCAATCAATTCGG |
| Suggested primer 2: | CGGTATGTACGCGGCTTTTG |