Insertion junction: CLIP2.043490_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.043490
Chromosome:chromosome 8
Location:1176579
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g363150 (1 of 2) PTHR23301//PTHR23301:SF0 - CHITIN BINDING PERITROPHIN-A // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTCATCACGGGCGCTACCAACGGCGGCACCTGCTGGAGGAACGACGTC
Internal bar code:TGGTGCTGGTTCCACGGATATC

Confirmation - LEAP-Seq

LEAP-Seq distance:3049
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GTTAATGCTACTGGCGTGCG
Suggested primer 2:CCGGTGAGTGAGGTTGAACA