| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.043494 |
| Chromosome: | chromosome 8 |
| Location: | 1395804 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364250 | (1 of 1) PF09174 - Maf1 regulator (Maf1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTCCCCCGGAGGGCTGGATGCGTGCGGGAGGAGGAAGGGAAACTCGA |
| Internal bar code: | CAACTGTTCAACTCGTTTCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1130 |
| LEAP-Seq percent confirming: | 54.5455 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTCGTCCTGGTTGTAGC |
| Suggested primer 2: | ATGAGATGGTGGCCTCTCCT |