Insertion junction: CLIP2.043498_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.043498
Chromosome:chromosome 17
Location:1919006
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g709900 (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATCGACAATCTGCGAAGCATCTTGACGCCGCCACCAAGCCCCATGCGCA
Internal bar code:GGGACACCACACTGGGGTGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:305
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCCTCCAGTCTTGCTTCC
Suggested primer 2:TGTACGAACAGGTCACAGGC