| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043517 |
| Chromosome: | chromosome 7 |
| Location: | 2417632 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328850 | CNK8 | NimA-related protein kinase; (1 of 1) PTHR24361:SF240 - PROTEIN MTK-1, ISOFORM B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGACTTTGGCCCGTGCGCGAGCACCCCGTGTTTTGGTAACCTGCCTGT |
| Internal bar code: | AATTCGGCTACAATTGGTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2216 |
| LEAP-Seq percent confirming: | 87.3134 |
| LEAP-Seq n confirming: | 117 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 134 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGTTGCAAGAGGGCATC |
| Suggested primer 2: | AACACGGTACCGGTGACATC |