Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.043547 |
Chromosome: | chromosome 2 |
Location: | 226419 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g800153 | intron | ||
Cre02.g800155 | CDS | ||
lncRNA_TCONS_00054465 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACAACCAGCCTCCAACACAATGCTCACCCACTTTTTGCTGAAACACAA |
Internal bar code: | CCCTATTTCGCTAACTAGTGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3439 |
LEAP-Seq percent confirming: | 95.7447 |
LEAP-Seq n confirming: | 45 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTACCGCTTACAGCCACCT |
Suggested primer 2: | TGTAGCAACCCCCGAGGATA |