| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.043575 |
| Chromosome: | chromosome 8 |
| Location: | 3053411 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375350 | SDR12 | (1 of 2) K19329 - WW domain-containing oxidoreductase (WWOX); Short-chain dehydrogenase/reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATCGAGGATTGGGCGGGAGCTTGGACTTAGCGGCCAGGAGTAGCGGC |
| Internal bar code: | GTGTCCTCAGGACGAACTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1777 |
| LEAP-Seq percent confirming: | 43.3333 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGTGTGCGTGTGTGTAT |
| Suggested primer 2: | TGTCCTTGCAGGGTGGTTTT |