| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043585 |
| Chromosome: | chromosome 6 |
| Location: | 7832986 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303400 | ADF3,FAP16 | (1 of 1) K18597 - echinoderm microtubule-associated protein-like 5 (EML5); necessary for axonemal severing | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAAACGGCGCGAGCATTGAAGGGTAGGCAGGCCAGCATATTTCCCTC |
| Internal bar code: | GCGGGGAAAGCGGTACAATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1101 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCGCCCCTAGAATCCAA |
| Suggested primer 2: | TCTTCTTCACCCACACGTCG |