| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.043592 |
| Chromosome: | chromosome 2 |
| Location: | 4795925 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108950 | LF1 | Long Flagella protein 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCGCTGTTGGCTCGCTGCAGCATCGCCGCCGCGGCCGCGGCCGTTGA |
| Internal bar code: | CAGCTCTGTGATTCAGATTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCCAATGCTGTCATTAGC |
| Suggested primer 2: | GACGACCGACCCTGAACTTT |