| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043597 |
| Chromosome: | chromosome 3 |
| Location: | 4614377 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176800 | FPG1,FPGS1 | (1 of 1) 6.3.2.12 - Dihydrofolate synthase / H(2)-folate synthetase; Putative folylpolyglutamate synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACATACACTGTCCTTCGTTCTTGCTTCCTCTTGCCTTCCCTTTAAACA |
| Internal bar code: | CCTCACCAAGACCTCTATAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1029 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCATCAGGGAGGGCATC |
| Suggested primer 2: | CCAACCACATGCCCACTTTG |