| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.043605 |
| Chromosome: | chromosome 8 |
| Location: | 891802 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361600 | (1 of 3) PF10250 - GDP-fucose protein O-fucosyltransferase (O-FucT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCGCCCAGCACCAGTTGCGGCTGCGTTTGCACAGACGCCCAGCCTGTC |
| Internal bar code: | GAGGGAGGTTGCATTTACTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2911 |
| LEAP-Seq percent confirming: | 97.7011 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCCAGGAGCTAGCTCAG |
| Suggested primer 2: | CAACGGGATAGGGTTACGGG |