Insertion junction: CLIP2.043609_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.043609
Chromosome:chromosome 16
Location:7083742
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g675301 (1 of 1) K13124 - mitogen-activated protein kinase organizer 1 (MORG1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTTGATGACCTGGATGGGGTCGATACTGCGTGACTTCATGTCCCACAC
Internal bar code:GATCTTGGAAGTCATGGCCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:363
LEAP-Seq percent confirming:72.2222
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CTACTGCTTGTCCTGTGGCA
Suggested primer 2:CTTCCATCTTGACCCGGCTT