Insertion junction: CLIP2.043617_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.043617
Chromosome:chromosome 12
Location:1555930
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g487601 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAGCCCCCATGCCGCGTGTAGCTGCTGCGACGTCTGTGCGCCGGCGTC
Internal bar code:CCGGCGCTGAATAGATACTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1813
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGATCGATGCCATGGCTA
Suggested primer 2:CAGGTGAGCGGCTCAAACTA