| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043651 |
| Chromosome: | chromosome 15 |
| Location: | 1307642 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641000 | NUP50 | Nucleoporin 50; (1 of 2) PF00638 - RanBP1 domain (Ran_BP1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGATTCACGAAAGGAACATGCGCACGCACGCACACAAACACATATCGA |
| Internal bar code: | TGGGCTTTAGTTGCGGTTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCCACGCATTTACAGCC |
| Suggested primer 2: | ACACACCTCTACACGCACAG |