Insertion junction: CLIP2.043662_4

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.043662
Chromosome:chromosome 15
Location:1726010
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCAAACTGGCGCAAACCGCGAGTGCCACGTATTATTAATTGCTATTACC
Internal bar code:TCTGGTGGCGCAGTCACCTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1289
LEAP-Seq percent confirming:41.6667
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CTAAATGTCGCGTTCGCCAG
Suggested primer 2:TGTCAGTTGCTGGTAGCTGG