| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.043665 |
| Chromosome: | chromosome 12 |
| Location: | 2835285 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACAGGATCACCTCGTTCGTGGACCGGGACATGCGGGTGGGCCGCGGCC |
| Internal bar code: | AATTGCGGTTTTCGTGGACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2725 |
| LEAP-Seq percent confirming: | 82.2222 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACCAACTCACTTGCG |
| Suggested primer 2: | CCTCGTGATCAGGTGCTGTT |