| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.043678 |
| Chromosome: | chromosome 2 |
| Location: | 7226491 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390949 | FAP140 | Flagellar Associated Protein 140 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATGCGAACGTGCCCCCGCCTTCTTAGAGCATCACCGCCGCCACTGCT |
| Internal bar code: | AACGCATCGCGCTGTAAAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2185 |
| LEAP-Seq percent confirming: | 89.7436 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCTGTGGATATGGAGGA |
| Suggested primer 2: | CCCAGGCATTCATGGTGTCT |