| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043727 |
| Chromosome: | chromosome 1 |
| Location: | 497805 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002950 | (1 of 1) PF06677 - Sjogren's syndrome/scleroderma autoantigen 1 (Autoantigen p27) (Auto_anti-p27) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCGCTTGTCAGTACAGGCGCAGGTGACACATTCCATGGCCAGTTCCG |
| Internal bar code: | ATTATGCTGATGCCAAACGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1144 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTATGGCTGGTGTTGCTCT |
| Suggested primer 2: | CGGGTAAGTATGGTCTCGGC |