Insertion junction: CLIP2.043733_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.043733
Chromosome:chromosome 10
Location:1732068
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g430150 REP27,LPA1 (1 of 1) PF11998//PF13414 - Protein of unknown function (DUF3493) (DUF3493) // TPR repeat (TPR_11); Low Photosystem II Accumulation 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAAGTCTGGAAGCAACAGGCAGTCCCCATTTTGTTTCTGAAGGGGCGG
Internal bar code:CGTATTTGAGTGACATTTCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:607
LEAP-Seq percent confirming:19.4444
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGGGAATGTTGCACTTT
Suggested primer 2:GTATTTGTGTGCGGGAGGGA