| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043737 |
| Chromosome: | chromosome 15 |
| Location: | 486699 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635950 | (1 of 2) PTHR10942 - LEISHMANOLYSIN-LIKE PEPTIDASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAATCCGCCTTGCAATAAGCCCCCCTCCCTTTCGATCCGCCCTGCATG |
| Internal bar code: | CCCTACAGAAGATGCTCGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 78 |
| LEAP-Seq percent confirming: | 12.0879 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCAACGAAAACGAGCGG |
| Suggested primer 2: | TTCGGGAACACACACACACA |