Insertion junction: CLIP2.043744_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.043744
Chromosome:chromosome 9
Location:1392842
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g400150 (1 of 2) PF13415//PF13418 - Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTACATGGATCCGGACTGCGGCGCGCCGCCGTACACATAGAGCGCCGC
Internal bar code:CGCAACGAAGGGCGATGTAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1080
LEAP-Seq percent confirming:51.5152
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTCCTGCACTGCTTTGAT
Suggested primer 2:CATGCCTCGAATCCCTCCTC