Insertion junction: CLIP2.043767_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.043767
Chromosome:chromosome 12
Location:9167675
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g543400 FDH1,GSNOR1 Nitrosoglutathione (GSNO) reductase 1; (1 of 2) 1.1.1.284 - S-(hydroxymethyl)glutathione dehydrogenase / NAD-linked formaldehyde dehydrogenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGTGCACACGTGCCGACGTTAATTGCCACGTGCTGCCACCACTGTCA
Internal bar code:GCCTAGTTACTACTAAAAAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:5514
LEAP-Seq percent confirming:52.1739
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:55
LEAP-Seq n unique pos:115

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCGCCTCGTTTGTTTTGC
Suggested primer 2:CAACTTGCCTTGCCACTGTC