| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043767 |
| Chromosome: | chromosome 12 |
| Location: | 9167675 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543400 | FDH1,GSNOR1 | Nitrosoglutathione (GSNO) reductase 1; (1 of 2) 1.1.1.284 - S-(hydroxymethyl)glutathione dehydrogenase / NAD-linked formaldehyde dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTGTGCACACGTGCCGACGTTAATTGCCACGTGCTGCCACCACTGTCA |
| Internal bar code: | GCCTAGTTACTACTAAAAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5514 |
| LEAP-Seq percent confirming: | 52.1739 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 115 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCGCCTCGTTTGTTTTGC |
| Suggested primer 2: | CAACTTGCCTTGCCACTGTC |