| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.043779 |
| Chromosome: | chromosome 3 |
| Location: | 7751047 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203451 | CYA5 | Adenylate cyclase; (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGTGCTATTCGCCTGACGTGCCTTGTTGTGTTGTGGTTGGGGTCGC |
| Internal bar code: | TCCCTTATTGCCACATAACGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1112 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCAGCCATGACGTTCCT |
| Suggested primer 2: | GCTGTGCTGGATCCAGACTT |