| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.043788 |
| Chromosome: | chromosome 9 |
| Location: | 2706449 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391650 | HCP4 | (1 of 4) 1.7.99.1 - Hydroxylamine reductase / Hydroxylamine (acceptor) reductase; Hybrid-cluster protein | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTCCCATTACAGGTTGCTTCACGTCACGCGGTCACACGACCTTCATC |
| Internal bar code: | ATACGCCCAAAGATACTTACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1177 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGGATTTTAGCGCCAGG |
| Suggested primer 2: | GGCTCTGGATAGCGATGGAC |