| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043793 |
| Chromosome: | chromosome 9 |
| Location: | 5955783 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412175 | (1 of 2) 3.7.1.8 - 2,6-dioxo-6-phenylhexa-3-enoate hydrolase / HOHPDA hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACCACCTCATCCAGACACACGCTGCACACCTCCACACACACACATAC |
| Internal bar code: | ATTTTCAGAAAGGTGTCGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 30.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTACACGCCCCGAATAT |
| Suggested primer 2: | CAGGAGGGCAGGTGGTTAAG |