| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043795 |
| Chromosome: | chromosome 13 |
| Location: | 439588 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g564850 | (1 of 1) PTHR31651:SF3 - AUXIN EFFLUX CARRIER FAMILY PROTEIN | 3'UTR | |
| Cre13.g801516 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTTCTGCCACTGCAACGATCAAGTACATGAATGCTGAACTGAGTAATG |
| Internal bar code: | CGTTAGGTTCCGAGGGTCTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1602 |
| LEAP-Seq percent confirming: | 95.3846 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTATAACCGACCGTCAG |
| Suggested primer 2: | CGCCAGTTCCGATTTTAGCG |