Insertion junction: CLIP2.043813_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.043813
Chromosome:chromosome 9
Location:886541
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g403400 (1 of 3) PF13637//PF13920 - Ankyrin repeats (many copies) (Ank_4) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCATAGACATGATCGTGCACACTGCTGCCACCCGCGGCAACGAATGA
Internal bar code:ATATAGTTCCAAGTTCTGTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3136
LEAP-Seq percent confirming:82.1429
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTGTTAGCATTGCGCATA
Suggested primer 2:GGCATACTCACCTGCACAGT