Insertion junction: CLIP2.043814_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.043814
Chromosome:chromosome 9
Location:1188886
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g401022 SNE3, UXE,UXE1 UDP-xylose-4-epimerase; (1 of 1) K12448 - UDP-arabinose 4-epimerase (UXE, uxe) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTTGGTTGGATTGTCCCTTCTTGGATCGTCGCAGTCATGACACCTGAG
Internal bar code:TTGGATCAGATCTCTTGGAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:301
LEAP-Seq percent confirming:51.6129
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAGGGAGGGAGGAAAGGC
Suggested primer 2:GAAAGAGGTTGGGGTCCAGG