| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.043823 |
| Chromosome: | chromosome 2 |
| Location: | 2796792 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094300 | FUO1 | (1 of 1) 1.5.5.1 - Electron-transferring-flavoprotein dehydrogenase / ETF-ubiquinone oxidoreductase; Electron transfer flavoprotein-ubiquinone oxidoreductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTATCATGGGATGCCATATGCTATAACGCAACCTTGCACTGAACCTCC |
| Internal bar code: | ACGTGGAGGTTTCAATGGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2369 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 121 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGATTCGTCGGGAACTCG |
| Suggested primer 2: | GTGTCGTCGACCTGAACCTT |