Insertion junction: CLIP2.043845_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.043845
Chromosome:chromosome 14
Location:2865315
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g627100 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGTCCGGGATCATCAGCGCCCGCGGCCGCTGCTGCTGCTGCTGCTCGT
Internal bar code:TTTCTATGAGGACGTCTCCTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:3289
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:GTACACGTTTCGCATCCTGC
Suggested primer 2:AGCGAAAACAGACTGTCCGT