| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.043852 |
| Chromosome: | chromosome 9 |
| Location: | 4852407 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402849 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCGCCGCATGAACCCCACCCCAGGTTCTGGCTCCCGACGTGCGCGCC |
| Internal bar code: | TTGGGGTTACTATACTAGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1514 |
| LEAP-Seq percent confirming: | 75.7576 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTCGGAAAATTCGGGGG |
| Suggested primer 2: | ATAACCCCAACCCCATGCTG |