Insertion junction: CLIP2.043852_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.043852
Chromosome:chromosome 9
Location:4852408
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g402849 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTGTTGTGATGGTGCAAAAATAAAAATCGCTCCACGCAGTCATAGATA
Internal bar code:TTGGGGTTACTATACTAGCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:79
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ATAACCCCAACCCCATGCTG
Suggested primer 2:GCATTCGGAAAATTCGGGGG