Insertion junction: CLIP2.043854_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.043854
Chromosome:chromosome 3
Location:6522805
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g194050 (1 of 274) IPR020683 - Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACGCAAAGGTAATACGGCCATTTCAGTAGATATGCTCAAGTTACGCT
Internal bar code:ATTAAAACTATATCGCTACACT

Confirmation - LEAP-Seq

LEAP-Seq distance:624
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TCCATACATGATCGCCTGCC
Suggested primer 2:GAAGCTGCGAGTCGTATCGA