| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043870 |
| Chromosome: | chromosome 6 |
| Location: | 3615007 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278135 | RPL21 | (1 of 1) K02889 - large subunit ribosomal protein L21e (RP-L21e, RPL21); Cytosolic 80S ribosomal protein L21 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCCCTCCAATCCGGGAACGCTGCAGTCCCCATGCCTTCGCTGCTGTG |
| Internal bar code: | TGAGAACAAGCGTGTTATTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2397 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCCACCAAGCTGCAAAG |
| Suggested primer 2: | CGTGGATGCGCTTCTTCATG |