Insertion junction: CLIP2.043877_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.043877
Chromosome:chromosome 12
Location:8664708
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g548051 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTCCACCGCTCGCATCGTCACAGCTGCGAACACAAGAACCATACCGGT
Internal bar code:TGGTTCCGACGCTAGATAATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:945
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AGCATACCTCGCTGATGTGG
Suggested primer 2:CTCAACCCACATCGTGTCCA