| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.043925 |
| Chromosome: | chromosome 7 |
| Location: | 657118 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317100 | POB18 | Proteome of basal body 18 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGGGGACCAATATCATGGACACACCCCAAACTCCTCTCATCCCTCAA |
| Internal bar code: | GCGGGGCGCCAACATTAGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 167 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAACCGCCTGTCTTGGTG |
| Suggested primer 2: | ATCCTCACTCCCCTTACCCC |