Insertion junction: CLIP2.043926_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.043926
Chromosome:chromosome 3
Location:8624123
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g210177 (1 of 3) PF13405//PF13499 - EF-hand domain (EF-hand_6) // EF-hand domain pair (EF-hand_7) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCACGGGGGCACCGGGTCGGCCAGCCGACTGTCCCAGACTGCTGTTTGC
Internal bar code:CGTTTCACGCGTACGAAGAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1253
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCTACCGAAGCAGATACC
Suggested primer 2:CTGCCTCACCTCTCCACATC