| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043952 |
| Chromosome: | chromosome 10 |
| Location: | 4474436 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450879 | (1 of 1) K14859 - ribosome biogenesis protein SSF1/2 (SSF1_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGTGCTATGATTGTGCCGGCTCGGCAGCGGAAGCCGGTCCTGGAGTTG |
| Internal bar code: | ACTTTCTTTTTATAAGTGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2219 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGCCGGACCCTAAGACT |
| Suggested primer 2: | CTAGTTGTGACCAGCGAGCA |