Insertion junction: CLIP2.043978_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.043978
Chromosome:chromosome 7
Location:1786450
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325725 (1 of 1) 1.1.1.47 - Glucose 1-dehydrogenase (NAD(P)(+)) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGGCTGATACTTACACCACCACCACCCCGCGCAGGTGGAGCAGCTGGG
Internal bar code:ATAGGATACTGAAACTCGCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:2538
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CCACGTTGAGCATGTCGTTC
Suggested primer 2:CACACAAGCAGAATGGCCAC