| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.043984 |
| Chromosome: | chromosome 6 |
| Location: | 7921978 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304050 | PKC1 | Protein Kinase C; (1 of 3) K15710 - E3 ubiquitin-protein ligase SHPRH [EC:3.6.4.- 6.3.2.19] (SHPRH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTGCACACCGCCCCGGCCGCCCAGTCGCATGTCGGGCCGGTGCCGCA |
| Internal bar code: | GAGGTTTGCGAAGAAGACCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2984 |
| LEAP-Seq percent confirming: | 82.8571 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAACTGTTGTACGGCCGT |
| Suggested primer 2: | TTGTCTTTTGAAGCTGGCGC |