| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.043986 |
| Chromosome: | chromosome 16 |
| Location: | 6242873 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676400 | HEL63 | (1 of 1) K12811 - ATP-dependent RNA helicase DDX46/PRP5 (DDX46, PRP5); DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAAGGCCGACCCACACGCACCTTGACCAGGCCGGCCTTGTGCTTGGTG |
| Internal bar code: | TCTAACTCTCAGTAACAGTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2110 |
| LEAP-Seq percent confirming: | 89.2857 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTGGTGGTGAACTACGA |
| Suggested primer 2: | GGATGTCGTCCTGTGTGTGT |