Insertion junction: CLIP2.043987_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.043987
Chromosome:chromosome 12
Location:8553505
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g549000 PHC4 Pherophorin-chlamydomonas homolog 4; (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCCGCAGGCCTGAGGATGGCGGAAAACGGCGGGCGGGCAAGACAGTCG
Internal bar code:TTAGTTTACAATTACACCTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3906
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGCGGCAGTATCAACATG
Suggested primer 2:GGTCCACCCAGACAGAGTTG