| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.044000 |
| Chromosome: | chromosome 2 |
| Location: | 2773018 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094050 | PIGO1,PIGO,PIG-O | (1 of 1) K05288 - phosphatidylinositol glycan, class O (PIGO); Ethanolamine transferase at GPI Mannose III | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGCATGAGGGCCTGCAACACCTTAGTGCGACCACCGCCCCGTGGCGT |
| Internal bar code: | TCGAACACTGTCACCCCGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1775 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTACGAGGACACGGTTGG |
| Suggested primer 2: | ACATCAACTGCTGACCGAGG |