| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044014 |
| Chromosome: | chromosome 2 |
| Location: | 5648654 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115650 | FBA4 | (1 of 2) PTHR11627//PTHR11627:SF20 - FRUCTOSE-BISPHOSPHATE ALDOLASE // FRUCTOSE-BISPHOSPHATE ALDOLASE; Fructose-1%252C6-bisphosphate aldolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCTGTCTGTTACCTCGCGCCCGTTCTTCATCGCGCTCATGCAGGGGCT |
| Internal bar code: | TTTATTAGATGTCATGGCCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3964 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGGGTGTGCAATGGTC |
| Suggested primer 2: | CAGCTGCTTCACCCTGAGAA |