| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.044021 |
| Chromosome: | chromosome 17 |
| Location: | 2829784 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718468 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTTGATCCAGTTGTTGGTGGTGAAGTTGGCAGCGGTGAGGTTGCCCC |
| Internal bar code: | GAATCGACCCTGGCCAACAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3480 |
| LEAP-Seq percent confirming: | 82.1429 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTACGTTTTGCCCCCTGG |
| Suggested primer 2: | GTCACCCGTATGCCGTCTAG |