Insertion junction: CLIP2.044030_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.044030
Chromosome:chromosome 7
Location:2749581
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g331300 AGP3 ADP-glucose pyrophosphorylase large subunit; (1 of 1) PF00132//PF00483 - Bacterial transferase hexapeptide (six repeats) (Hexapep) // Nucleotidyl transferase (NTP_transferase) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCGGCCGCAGCTGCACACCCGCCGCCGCCGCCCGCTCCTGCCGCTCCT
Internal bar code:GATTTACTGGACGCATCATACG

Confirmation - LEAP-Seq

LEAP-Seq distance:352
LEAP-Seq percent confirming:94.4444
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CACACAATTCCACAGCCACG
Suggested primer 2:CCACACACACACACACACAC