| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.044040 |
| Chromosome: | chromosome 10 |
| Location: | 4715351 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCTGGGGGCATCCTCGGGAGCGGGATATTGCAGATGTCGGCTCCCGGG |
| Internal bar code: | AAACGTCTCTGTAAACCGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3032 |
| LEAP-Seq percent confirming: | 68.4211 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTACAGACACAAGGCAG |
| Suggested primer 2: | CTCACAGTTTGTCGCCTCCT |