| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.044070 |
| Chromosome: | chromosome 6 |
| Location: | 6646116 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294850 | UBC10 | Ubiquitin-conjugating enzyme E2; (1 of 1) PTHR24067:SF142 - UBIQUITIN-CONJUGATING ENZYME E2 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTGAACTTGACAGTGGGCGCCTTATTAGGGTACTCCTCCGAGAACTC |
| Internal bar code: | GAATGCTAGACTGGCGCAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3521 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAATCCGCCAGCGATTG |
| Suggested primer 2: | CCACGCTTTCAACACATGCA |