| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.044109 |
| Chromosome: | chromosome 7 |
| Location: | 4655987 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344702 | (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCCAGGTACGTCAGGCGCCTGCAGCCTACACCCGCTCCTGCACTCAC |
| Internal bar code: | GATTTTAACTAAGTAAACGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGTGGGATGAGCGGGAG |
| Suggested primer 2: | AAGAGTTTCAGGCACTCGGG |