Insertion junction: CLIP2.044113_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.044113
Chromosome:chromosome 17
Location:3135905
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g720700 ANK14 Predicted protein with ankyrin repeats; (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGCGCTGTTGGACGCCGGCGCCACACCCAACGCCCCCTTCTTCGAGGT
Internal bar code:CGTCCGGGAGGGTTTGACGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:167
LEAP-Seq percent confirming:6.66667
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GACTGTTCAGAAGACGGCGA
Suggested primer 2:GCAGCGCCTTACTACCGTAT